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mouse anti cd66b  (Novus Biologicals)


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    Novus Biologicals mouse anti cd66b
    Bone tissues surgically removed from PJI patient with S. aureus osteomyelitis were processed for histology and immunohistochemistry. (A) Representative 100x image (bar =100 μm) of a H&E-stained section is shown to illustrate the inflammatory cells within the region of interest (box). ( B-D) Parallel histology sections containing the region of interest were immunostained with labelled antibodies against CD3, PD1, S. aureus, TIM-3 (green), LAG-3, and <t>CD66b,</t> counter stained with DAPI, and representative fluorescent microscopy images are shown at 200x (bar = 100 μm). ( B ) Note CD3 + /PD1 + T cells detected in areas of S. aureus infection (white arrows). (C) Note CD3 + /TIM-3 + (white arrows) and CD3 + /LAG-3 + (yellow arrows) T cells at the site of S. aureus infection. (D) Note TIM-3 + /CD66b+ neutrophils at the site of infection (white arrows).
    Mouse Anti Cd66b, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+ceacam8+cd66b/CEACAM8%2FCD66b+Antibody+(G10F5)+-+Azide+and+BSA+Free/bio_rxiv__2024__12__30__630837-189-49-54
    Average 93 stars, based on 3 article reviews
    mouse anti cd66b - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Immune Checkpoint Molecules as Biomarkers of Staphylococcus aureus Bone Infection and Clinical Outcome"

    Article Title: Immune Checkpoint Molecules as Biomarkers of Staphylococcus aureus Bone Infection and Clinical Outcome

    Journal: bioRxiv

    doi: 10.1101/2024.12.30.630837

    Bone tissues surgically removed from PJI patient with S. aureus osteomyelitis were processed for histology and immunohistochemistry. (A) Representative 100x image (bar =100 μm) of a H&E-stained section is shown to illustrate the inflammatory cells within the region of interest (box). ( B-D) Parallel histology sections containing the region of interest were immunostained with labelled antibodies against CD3, PD1, S. aureus, TIM-3 (green), LAG-3, and CD66b, counter stained with DAPI, and representative fluorescent microscopy images are shown at 200x (bar = 100 μm). ( B ) Note CD3 + /PD1 + T cells detected in areas of S. aureus infection (white arrows). (C) Note CD3 + /TIM-3 + (white arrows) and CD3 + /LAG-3 + (yellow arrows) T cells at the site of S. aureus infection. (D) Note TIM-3 + /CD66b+ neutrophils at the site of infection (white arrows).
    Figure Legend Snippet: Bone tissues surgically removed from PJI patient with S. aureus osteomyelitis were processed for histology and immunohistochemistry. (A) Representative 100x image (bar =100 μm) of a H&E-stained section is shown to illustrate the inflammatory cells within the region of interest (box). ( B-D) Parallel histology sections containing the region of interest were immunostained with labelled antibodies against CD3, PD1, S. aureus, TIM-3 (green), LAG-3, and CD66b, counter stained with DAPI, and representative fluorescent microscopy images are shown at 200x (bar = 100 μm). ( B ) Note CD3 + /PD1 + T cells detected in areas of S. aureus infection (white arrows). (C) Note CD3 + /TIM-3 + (white arrows) and CD3 + /LAG-3 + (yellow arrows) T cells at the site of S. aureus infection. (D) Note TIM-3 + /CD66b+ neutrophils at the site of infection (white arrows).

    Techniques Used: Immunohistochemistry, Staining, Microscopy, Infection

    Related Articles

    Incubation:

    Article Title: Electrical stimulation of the vagus nerve ameliorates inflammation and disease activity in a rat EAE model of multiple sclerosis.
    Article Snippet: .. Sections were incubated with respective primary antibodies such as Alexa- 488 conjugated mouse anti- rat CD4 (domain 1, Cat #MCA55A488, Biorad, Hercules, CA); eFluor660 conjugated mouse anti- rat IFN- γ (clone DB1, Cat #50- 7310- 80, Invitrogen, Carlsbad, CA); goat anti- IL- 17 (Cat #SC6078, Dallas, TX); sheep anti- fibrinogen (Cat #F4203- 02F, US Biologicals, Salem, MA); Alexa- 488 conjugated anti- GFAP (Cat #53- 9892- 82, Invitrogen, Carlsbad, CA), Alexa- 488 conjugated claudin- 5 (Cat #352588, Thermo Fisher Scientific, Waltham, MA), rabbit anti- CD31(Cat #PA5- 32321, Thermo Fisher Scientific, Waltham, MA); mouse anti- Iba1 antibody (Cat #ab283319, Abcam, Waltham, MA); Alexa- 568 conjugated rabbit anti- iNOS (Cat #Ab20995, Abcam, Waltham, MA); Alexa- 647 conjugated rabbit anti- CD206 (Cat #Ab195192, Abcam, Waltham, MA); Alexa- 647 conjugated rabbit anti- CD31 (Cat #Ab218582, Abcam, Waltham, MA); and mouse anti- CEACAM8/CD66b (Cat #NB100- 77808, Novus Biological LLC, Centennial, CO) at 1:100 concentration in 1% normal goat serum at 4 °C overnight. ..

    Article Title: Electrical stimulation of the vagus nerve ameliorates inflammation and disease activity in a rat EAE model of multiple sclerosis
    Article Snippet: .. Sections were incubated with respective primary antibodies such as Alexa-488 conjugated mouse anti-rat CD4 (domain 1, Cat #MCA55A488, Biorad, Hercules, CA); eFluor660 conjugated mouse anti-rat IFN-γ (clone DB1, Cat #50-7310-80, Invitrogen, Carlsbad, CA); goat anti-IL-17 (Cat #SC-6078, Dallas, TX); sheep anti-fibrinogen (Cat #F4203-02F, US Biologicals, Salem, MA); Alexa-488 conjugated anti-GFAP (Cat #53-9892-82, Invitrogen, Carlsbad, CA), Alexa-488 conjugated claudin-5 (Cat #352588, Thermo Fisher Scientific, Waltham, MA), rabbit anti-CD31(Cat #PA5-32321, Thermo Fisher Scientific, Waltham, MA); mouse anti-Iba1 antibody (Cat #ab283319, Abcam, Waltham, MA); Alexa-568 conjugated rabbit anti-iNOS (Cat #Ab20995, Abcam, Waltham, MA); Alexa-647 conjugated rabbit anti-CD206 (Cat #Ab195192, Abcam, Waltham, MA); Alexa-647 conjugated rabbit anti-CD31 (Cat #Ab218582, Abcam, Waltham, MA); and mouse anti-CEACAM8/CD66b (Cat #NB100-77808, Novus Biological LLC, Centennial, CO) at 1:100 concentration in 1% normal goat serum at 4 °C overnight. ..

    Concentration Assay:

    Article Title: Electrical stimulation of the vagus nerve ameliorates inflammation and disease activity in a rat EAE model of multiple sclerosis.
    Article Snippet: .. Sections were incubated with respective primary antibodies such as Alexa- 488 conjugated mouse anti- rat CD4 (domain 1, Cat #MCA55A488, Biorad, Hercules, CA); eFluor660 conjugated mouse anti- rat IFN- γ (clone DB1, Cat #50- 7310- 80, Invitrogen, Carlsbad, CA); goat anti- IL- 17 (Cat #SC6078, Dallas, TX); sheep anti- fibrinogen (Cat #F4203- 02F, US Biologicals, Salem, MA); Alexa- 488 conjugated anti- GFAP (Cat #53- 9892- 82, Invitrogen, Carlsbad, CA), Alexa- 488 conjugated claudin- 5 (Cat #352588, Thermo Fisher Scientific, Waltham, MA), rabbit anti- CD31(Cat #PA5- 32321, Thermo Fisher Scientific, Waltham, MA); mouse anti- Iba1 antibody (Cat #ab283319, Abcam, Waltham, MA); Alexa- 568 conjugated rabbit anti- iNOS (Cat #Ab20995, Abcam, Waltham, MA); Alexa- 647 conjugated rabbit anti- CD206 (Cat #Ab195192, Abcam, Waltham, MA); Alexa- 647 conjugated rabbit anti- CD31 (Cat #Ab218582, Abcam, Waltham, MA); and mouse anti- CEACAM8/CD66b (Cat #NB100- 77808, Novus Biological LLC, Centennial, CO) at 1:100 concentration in 1% normal goat serum at 4 °C overnight. ..

    Article Title: Electrical stimulation of the vagus nerve ameliorates inflammation and disease activity in a rat EAE model of multiple sclerosis
    Article Snippet: .. Sections were incubated with respective primary antibodies such as Alexa-488 conjugated mouse anti-rat CD4 (domain 1, Cat #MCA55A488, Biorad, Hercules, CA); eFluor660 conjugated mouse anti-rat IFN-γ (clone DB1, Cat #50-7310-80, Invitrogen, Carlsbad, CA); goat anti-IL-17 (Cat #SC-6078, Dallas, TX); sheep anti-fibrinogen (Cat #F4203-02F, US Biologicals, Salem, MA); Alexa-488 conjugated anti-GFAP (Cat #53-9892-82, Invitrogen, Carlsbad, CA), Alexa-488 conjugated claudin-5 (Cat #352588, Thermo Fisher Scientific, Waltham, MA), rabbit anti-CD31(Cat #PA5-32321, Thermo Fisher Scientific, Waltham, MA); mouse anti-Iba1 antibody (Cat #ab283319, Abcam, Waltham, MA); Alexa-568 conjugated rabbit anti-iNOS (Cat #Ab20995, Abcam, Waltham, MA); Alexa-647 conjugated rabbit anti-CD206 (Cat #Ab195192, Abcam, Waltham, MA); Alexa-647 conjugated rabbit anti-CD31 (Cat #Ab218582, Abcam, Waltham, MA); and mouse anti-CEACAM8/CD66b (Cat #NB100-77808, Novus Biological LLC, Centennial, CO) at 1:100 concentration in 1% normal goat serum at 4 °C overnight. ..

    Staining:

    Article Title: Staphylococcus aureus Panton-Valentine Leukocidin worsens acute implant-associated osteomyelitis in humanized BRGSF mice
    Article Snippet: Higher magnification (40X) images were acquired with Olympus BX40 light microscope. .. The following primary antibodies were used for immunofluorescent staining of HuBRGSF infected limbs (USA300 WT, USA300 Δpvl, or USA300 Δpvl+pvl): mouse anti-CEACAM8/CD66b for neutrophils (Clone G10F5, NBP2–80664, Novus Biologicals, Zug, Switzerland), rabbit anti- S. aureus (PA1–7246, RRID:AB_561546, Thermo Fisher Scientific), and mouse anti-CD68 for monocytes (Clone PG-M1, MS-1808-S1, RRID:AB_149350, Thermo Fisher Scientific). ..

    Infection:

    Article Title: Staphylococcus aureus Panton-Valentine Leukocidin worsens acute implant-associated osteomyelitis in humanized BRGSF mice
    Article Snippet: Higher magnification (40X) images were acquired with Olympus BX40 light microscope. .. The following primary antibodies were used for immunofluorescent staining of HuBRGSF infected limbs (USA300 WT, USA300 Δpvl, or USA300 Δpvl+pvl): mouse anti-CEACAM8/CD66b for neutrophils (Clone G10F5, NBP2–80664, Novus Biologicals, Zug, Switzerland), rabbit anti- S. aureus (PA1–7246, RRID:AB_561546, Thermo Fisher Scientific), and mouse anti-CD68 for monocytes (Clone PG-M1, MS-1808-S1, RRID:AB_149350, Thermo Fisher Scientific). ..



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    Bone tissues surgically removed from PJI patient with S. aureus osteomyelitis were processed for histology and immunohistochemistry. (A) Representative 100x image (bar =100 μm) of a H&E-stained section is shown to illustrate the inflammatory cells within the region of interest (box). ( B-D) Parallel histology sections containing the region of interest were immunostained with labelled antibodies against CD3, PD1, S. aureus, TIM-3 (green), LAG-3, and <t>CD66b,</t> counter stained with DAPI, and representative fluorescent microscopy images are shown at 200x (bar = 100 μm). ( B ) Note CD3 + /PD1 + T cells detected in areas of S. aureus infection (white arrows). (C) Note CD3 + /TIM-3 + (white arrows) and CD3 + /LAG-3 + (yellow arrows) T cells at the site of S. aureus infection. (D) Note TIM-3 + /CD66b+ neutrophils at the site of infection (white arrows).
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    Image Search Results


    Bone tissues surgically removed from PJI patient with S. aureus osteomyelitis were processed for histology and immunohistochemistry. (A) Representative 100x image (bar =100 μm) of a H&E-stained section is shown to illustrate the inflammatory cells within the region of interest (box). ( B-D) Parallel histology sections containing the region of interest were immunostained with labelled antibodies against CD3, PD1, S. aureus, TIM-3 (green), LAG-3, and CD66b, counter stained with DAPI, and representative fluorescent microscopy images are shown at 200x (bar = 100 μm). ( B ) Note CD3 + /PD1 + T cells detected in areas of S. aureus infection (white arrows). (C) Note CD3 + /TIM-3 + (white arrows) and CD3 + /LAG-3 + (yellow arrows) T cells at the site of S. aureus infection. (D) Note TIM-3 + /CD66b+ neutrophils at the site of infection (white arrows).

    Journal: bioRxiv

    Article Title: Immune Checkpoint Molecules as Biomarkers of Staphylococcus aureus Bone Infection and Clinical Outcome

    doi: 10.1101/2024.12.30.630837

    Figure Lengend Snippet: Bone tissues surgically removed from PJI patient with S. aureus osteomyelitis were processed for histology and immunohistochemistry. (A) Representative 100x image (bar =100 μm) of a H&E-stained section is shown to illustrate the inflammatory cells within the region of interest (box). ( B-D) Parallel histology sections containing the region of interest were immunostained with labelled antibodies against CD3, PD1, S. aureus, TIM-3 (green), LAG-3, and CD66b, counter stained with DAPI, and representative fluorescent microscopy images are shown at 200x (bar = 100 μm). ( B ) Note CD3 + /PD1 + T cells detected in areas of S. aureus infection (white arrows). (C) Note CD3 + /TIM-3 + (white arrows) and CD3 + /LAG-3 + (yellow arrows) T cells at the site of S. aureus infection. (D) Note TIM-3 + /CD66b+ neutrophils at the site of infection (white arrows).

    Article Snippet: Primary antibodies: The following antibodies were utilized for immunostaining: goat anti-CD3ε (clone M-20, sc-1127, RRID:AB_631128, Santa Cruz Biotechnology), mouse anti-PD-1 (10377-MM23, RRID:AB_2936309, Sino Biologicals), Rabbit anti-LAG3 (clone BLR027F, NBP2-76402, RRID:AB_3403543, Novus Biologicals), Mouse anti-TIM3/HAVCR2 (clone TIM3/4031, V8754-20UG, NSJ Bioreagents), Rabbit anti- S. aureus (PA1-7246, RRID:AB_561546, Thermo Fisher Scientific), and Mouse anti-CD66b (G10F5, NBP2-80664, RRID:AB_3096017, Novus Biologicals).

    Techniques: Immunohistochemistry, Staining, Microscopy, Infection